Steps for Using a HBV Test
1. Sample Collection & Preparation
Collect whole blood, serum, or plasma via venipuncture or fingerstick (depending on test type).
For rapid tests, use provided capillary tubes or droppers to transfer 10-50 µL of sample.
For ELISA/PCR, centrifuge blood samples (if required) and aliquot into sterile tubes.
2. Test Setup
A. Rapid Lateral Flow Test:
Place the test cassette on a flat, dry surface.
Add 3-4 drops of sample (or buffer if specified) into the sample well.
Wait 10-15 minutes for visible lines (control + result).
B. ELISA Procedure:
Load samples, standards, and controls into pre-coated microplate wells.
Incubate (30-60 min at 37°C), wash, and add enzyme conjugate.
After further incubation, add TMB substrate for color development.
Stop the reaction and measure absorbance with a microplate reader.
C. PCR Testing:
Extract HBV DNA/RNA using the provided kit.
Prepare the PCR mix (primers, probes, master mix) and load into reaction tubes.
Run real-time PCR with recommended cycling conditions.
3. Interpretation of Results
Rapid Test:
Positive: Control line (C) + test line (T) visible.
Negative: Only control line (C) appears.
Invalid: No control line – repeat test.
ELISA/PCR:
Compare optical density (OD) or Ct values against cutoff standards.
Quantify viral load (if applicable).
4. Post-Test Protocol
Dispose of biohazard waste properly.
Record results and report as per clinical guidelines.
Note: Always refer to the product insert for specific instructions.
