Steps for Using an HBsAg Test
1. Sample Collection & Preparation
Collect whole blood, serum, or plasma via venipuncture or fingerstick (depending on test type).
For rapid tests, use the provided capillary tubes or droppers to transfer 10-50 µL of the sample.
For ELISA/PCR, centrifuge blood samples (if required) and aliquot into sterile tubes.
2. Test Setup
Place the test cassette on a flat, dry surface.
Add 3-4 drops of sample (or buffer if specified) into the sample well.
Wait 10-15 minutes for visible lines (control + result).
Load samples, standards, and controls into pre-coated microplate wells.
Incubate (30-60 min at 37°C), wash, and add enzyme conjugate.
After further incubation, add TMB substrate for color development.
Stop the reaction and measure absorbance with a microplate reader.
Extract HBsAg Test DNA/RNA using the provided kit.
Prepare the PCR mix (primers, probes, master mix) and load it into the reaction tubes.
Run real-time PCR with recommended cycling conditions.
3. Interpretation of Results
Positive: Control line (C) + test line (T) visible.
Negative: Only the control line (C) appears.
Invalid: No control line – repeat test.
Compare optical density (OD) or Ct values against cutoff standards.
Quantify viral load (if applicable).
4. Post-Test Protocol
Dispose of biohazard waste properly.
Record results and report as per clinical guidelines.
Note: Always refer to the product insert for specific instructions.
Why Choose Our HBsAg Test?
Early detection reduces the risk of HBV complications.
Cost-effective with minimal equipment requirements (for rapid tests).
Scalable for high-throughput testing in laboratories.
The HBsAg Test is an essential tool in global efforts to eliminate Hepatitis B.
The HBsAg Test is a vital tool in global efforts to eliminate Hepatitis B.
Features of HBsAg Test
1. Superior Diagnostic Performance & Accuracy
Ultra-Sensitive Detection:
Utilizes optimized monoclonal/polyclonal antibody pairs for detection levels as low as<0.1 IU/mL.
Ensures equal efficiency in detecting all major genotypes (A-H).
Features a unique pre-treatment step to dissociate immune complexes, significantly reducing false negatives.
Exceptional Specificity & Interference Resistance:
Demonstrates<0.01% cross-reactivity with HIV/HCV antibodies, Rheumatoid Factor (RF), and Human Anti-Mouse Antibodies (HAMA).
Internal hook effect prevention up to 500,000 IU/mL ensures accurate results for high-titer samples.
Dual-wavelength reading (450/630nm) minimizes background interference for cleaner signals.
Advanced Capabilities for Complex Cases:
Capable of identifying mutant HBsAg, crucial for detecting vaccine breakthrough infections.
Validated for Dried Blood Spot (DBS) testing, simplifying sample collection and transport.
Offers both quantitative and semi-quantitative reporting options to meet diverse clinical needs.
2. Optimized for Laboratory Workflow & Efficiency
3. Summary: A New Standard in HBsAg Testing
This fourth-generation HBsAg assay combines diagnostic accuracy with operational excellence, outperforming conventional tests in:
✓ Earlier Detection: Identifies infections 5-7 days earlier, closing the diagnostic window period.
✓ Broader Coverage: Reliably detects immune escape variants often missed by other assays.
✓ Enhanced Throughput: Fully optimized for high-throughput, automated laboratory workflows.
